Coding

Part:BBa_K4694009:Design

Designed by: Becky Davies   Group: iGEM23_Exeter   (2023-09-29)


Sp-His-QQ-5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 759
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1138
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence has been mutated to remove forbidden restriction sites to be compatible with BioBrick and Type IIS assembly.


Source

Isolated during genomic screening.

References